SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Methylome analysis using MeDIP-seq with low DNA concentrations. Newsbot! Literature Watch 1 04-25-2017 05:06 AM
Trying to identify low coverage regions (bases) in my sample from a Bam and BED file. pfeifferr Bioinformatics 4 03-24-2014 12:22 PM
low 260/230 ratio for RNA-seq sample yuzhang Sample Prep / Library Generation 3 09-27-2012 01:20 PM
Alternating High/Low intensity of sample, but not PhiX BBthekid007 Illumina/Solexa 2 02-22-2012 07:36 AM
Low Molecular Weight sample and primer dimers jsandler 454 Pyrosequencing 1 08-26-2009 02:27 AM

Reply
 
Thread Tools
Old 10-10-2018, 06:26 AM   #1
smoics
Junior Member
 
Location: US

Join Date: Jan 2016
Posts: 7
Default low ending sample concentrations

My sample concentrations post-library prep are a lot lower using the Flex DNA kit compared to what I've had in the past using the XT kit. Is this likely loss during bead clean up (as this kit has more bead steps than the other kit)? Drying out of beads before resuspending into PCR mix? Is there any need to add additional PCR cycles, on top of their recommendation?

This could just be a fluke from my first prep using this kit, but I'd like to know how to improve before repeating. Thanks!
smoics is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:08 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO