Is anyone aware of NGS-based estimates of mutation rates for mammalian cell culture? I know that NGS was used to estimate germline mutation rate in humans but I am interested in the mutation rate of human cells in culture. So this would be somatic/mitotic mutation rate.
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I have no idea on studies using NGS, but some independent OLD papers suggests in vitro cell culture increase mutation rate by 10x
in vivo retionblastoma: 10x-8 order
(http://www.ncbi.nlm.nih.gov/pmc/arti...00396-0056.pdf)
in vivo Y chromosome
in vitro germlien: 10x-8, approaching 10-7
(http://www.pnas.org/content/89/15/7036.full.pdf)
Normal vs Malignant lymphocyte, 10x in malignant
(http://cancerres.aacrjournals.org/co...2/407.full.pdf)
Somatic mutation: 10-7
(http://cancerres.aacrjournals.org/co.../18/8111.short)Marco
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Originally posted by marcowanger View PostI have no idea on studies using NGS, but some independent OLD papers suggests in vitro cell culture increase mutation rate by 10x
in vivo retionblastoma: 10x-8 order
(http://www.ncbi.nlm.nih.gov/pmc/arti...00396-0056.pdf)
in vivo Y chromosome
in vitro germlien: 10x-8, approaching 10-7
(http://www.pnas.org/content/89/15/7036.full.pdf)
Normal vs Malignant lymphocyte, 10x in malignant
(http://cancerres.aacrjournals.org/co...2/407.full.pdf)
Somatic mutation: 10-7
(http://cancerres.aacrjournals.org/co.../18/8111.short)
Thanks.
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Originally posted by genericforms View PostThere is a recent nextgen study that puts human y-chromosome mutation rate at 10^-8 but I too have not found a NGS-derived cell culture mutation rate. So I suppose this does put cell culture at 10X the mutation rate of the human germline.
Thanks.Marco
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