Dear users,
I would like to use directional RNA sequencing protocol (with TrueSeq siRNA adapters) to prepare libraries from fragmented RNA of purified virus. After the fragmentation and end repair I`ve got only around 1 ng of RNA (depending on Bioanalyzer quantification).
So, the question is: do I have chances to get a library from such a low input and does someone have experinces with low input in directional RNA sequencing library preparation? Would I need to adjust (lower) the concentration of adapters? I would be happy for any remarks.
Thank you very much!
I would like to use directional RNA sequencing protocol (with TrueSeq siRNA adapters) to prepare libraries from fragmented RNA of purified virus. After the fragmentation and end repair I`ve got only around 1 ng of RNA (depending on Bioanalyzer quantification).
So, the question is: do I have chances to get a library from such a low input and does someone have experinces with low input in directional RNA sequencing library preparation? Would I need to adjust (lower) the concentration of adapters? I would be happy for any remarks.
Thank you very much!
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