Pac Bio Proof-of-Concept Science Paper on Single-Molecule Sequencing Technology

[Digi]

Pacific Biosciences published a proof-of-concept study at online today in Science.

Below is detailed news from Genomeweb:

http://www.genomeweb.com/issues/news/150855-1.html

And the article is linked below:

http://www.sciencemag.org/cgi/content/abstract/1162986

[monad]

If this is working as described with improvement, I believe this would be the next-next gen break-through technology.

[Fred]

Hi,
PacBio said that they have sequenced the genome of E. coli and have obtained a 3,200-base read that spans a large repeat region.
source : http://www.genomeweb.com/sequencing

Fred

[pohyg]

pac bio on TV.

http://www.youtube.com/watch?v=zJ4XafX5_UU

[Fred]

Hi,

Some news about PacBio :
http://www.forbes.com/forbes/2009/10...e-machine.html

http://www.genomeweb.com/sequencing/...rna-sequencing

Fred

[Chipper]

Here is some news from PacBio:

http://www.scivee.tv/node/11409

[RockChalkJayhawk]

Questions about PacBio

Hey all.

I have a couple of questions about Pacific Biosciences that some of you might be able to answer.

Q1: Does anyone have any insight as to who the "early access" people are for PacBio's SMRT sequencers? So far I've heard of Scripps and Monsanto, but there are also genome centers and academic centers. (I would guess WashU would get one).

Q2: If the cameras are limiting the throughput, then how many ZMWs are functioning in the 1st generation instruments?

Q3: The last I heard, the DNA polymerase was getting about 4.63 bp/s, giving 400kb/ZMW/day which translates into 1.7X coverage of the human genome per day. Is there an update on this yet?

Q4: I have a ton of people that have been asking me about this technology (I guess I'm the designated sequencing guru at my university). Does anyone have a clue on how the company wants to choose the places it sells the sequencers to (and how many it will be able to manufacture at the time of release)? I know there is a long list of people itching to get thier hands on it (drug companies, genome centers, etc), and I'm trying to find a way to get one at our medical center. Any ideas on how to get to the top of the list? ( I think they are pushing for cancer and infectious diseases, but I also read they want to diversify thier clientel). Any thoughts?

Q5: How come these sequencers haven't been named yet????