Hi all,
I have 454 *.sff file and successfully converted it into *.fasta and *.qaul using PyroBayes.
From these *.fasta and *.qual files i am generating *.fastq file by a perl script fastaQual2fastq.pl, but when i try to run MosaikBuild, it gives error : can not open FASTQ file, integrity check failed.
Why? Pls help me.
I have attached perl file with this.
I am running Mosaik on ubuntu 9.10.
I have 454 *.sff file and successfully converted it into *.fasta and *.qaul using PyroBayes.
From these *.fasta and *.qual files i am generating *.fastq file by a perl script fastaQual2fastq.pl, but when i try to run MosaikBuild, it gives error : can not open FASTQ file, integrity check failed.
Why? Pls help me.
I have attached perl file with this.
I am running Mosaik on ubuntu 9.10.
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