Hi all
I have an Illumina PE library that is of very low concentration (~1.5nM). If I speedvac I should be able to get 2nM to do the denaturation, but I'll have to do it in 10µL rather than the stated 20µL as I don't have the volume. Has anyone done this before?
Thanks
I have an Illumina PE library that is of very low concentration (~1.5nM). If I speedvac I should be able to get 2nM to do the denaturation, but I'll have to do it in 10µL rather than the stated 20µL as I don't have the volume. Has anyone done this before?
Thanks
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