Hi, I select 11 microsatellites generated by MISA program (http://pgrc.ipk-gatersleben.de/misa/), now I want to design primer, and I use this.
But the outgoing message is
I chek the *.misa file, all is OK, but the problem is with primer3_core (I suppose) with the message "Use of uninitialized value $count in concatenation (.) or string at ./p3_out.pl line 76" and in the end with p3_out.pl the *.result show the SSR, SSR type but not the primers sequences.
PD: I want to use the program and not the online service because after I have to do the same proces but with a genome and selec more than 100 microsatellites.
Code:
misa.pl FASTA.fa p3_in.pl FASTA.fa.misa primer3_core < FASTA.fa.p3in > FASTA.fa.p3out p3_out.pl FASTA.fa.p3out FASTA.fa.misa
Code:
11 record was created. Use of uninitialized value $count in concatenation (.) or string at ./p3_out.pl line 76, <SRC> chunk 11. Primer modelling was succefull for sequences. Primer modelling failed for 11 sequences.
PD: I want to use the program and not the online service because after I have to do the same proces but with a genome and selec more than 100 microsatellites.
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