Hi,
I've been trying to de-multiplex my sequencing data using the interface on Galaxy. However I keep getting the following error message.
Use of uninitialized value $barcode in uc at /home/g2main/linux3.2-x86_64/bin/fastx_barcode_splitter.pl line 156, <BCFILE> line 10.
Error: bad barcode value () at barcode file (/galaxy/main_pool/pool4/files/005/946/dataset_5946981.dat) line 10
gzip: stdout: Broken pipe
The barcode file I've used include.
#Barcode
BC1 GATCAG
BC2 TTAGGC
BC3 TAGCTT
BC4 TGACCA
BC5 GGCTAC
BC6 ACAGTG
BC7 CTTGTA
BC8 GCCAAT
-mismatch i've tried 0,1,and 2 but none of them works.
The fastq raw files (illumina platform) I've uploaded does not qualify for splitting, so i've groomed it to fastqsanger.
Any suggestion?
I've been trying to de-multiplex my sequencing data using the interface on Galaxy. However I keep getting the following error message.
Use of uninitialized value $barcode in uc at /home/g2main/linux3.2-x86_64/bin/fastx_barcode_splitter.pl line 156, <BCFILE> line 10.
Error: bad barcode value () at barcode file (/galaxy/main_pool/pool4/files/005/946/dataset_5946981.dat) line 10
gzip: stdout: Broken pipe
The barcode file I've used include.
#Barcode
BC1 GATCAG
BC2 TTAGGC
BC3 TAGCTT
BC4 TGACCA
BC5 GGCTAC
BC6 ACAGTG
BC7 CTTGTA
BC8 GCCAAT
-mismatch i've tried 0,1,and 2 but none of them works.
The fastq raw files (illumina platform) I've uploaded does not qualify for splitting, so i've groomed it to fastqsanger.
Any suggestion?
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