I am using Agilent SureSelect XT for targeted enrichment. The provided protocol describes preforming the hyb wash in 1.5ml tubes. I will be needing to prepare 48 or more samples at a time. I need to do this manually as we do not have automation in our lab yet. Has anyone preformed the hyb wash in a 96-well plate? I assuming you would need decrease the volume of buffer and increase the number of washes, but any help or example where this has been successful would be greatly appreciated!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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