Hi All!
I downloaded V1-V2 hypervariable region of 16S sequence data of Fusobacterium sequenced by 454. I was expecting taht all the reads to be identical as it is one gene of single species but the reads are different. I want to know if this should be the case that there is variability in the sequences of single gene or all the sequences should be identical.
Any kind of help will be appreciated.
Thanks!!
I downloaded V1-V2 hypervariable region of 16S sequence data of Fusobacterium sequenced by 454. I was expecting taht all the reads to be identical as it is one gene of single species but the reads are different. I want to know if this should be the case that there is variability in the sequences of single gene or all the sequences should be identical.
Any kind of help will be appreciated.
Thanks!!
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