Hi All
I am try to align RNAseq data to a genome to map the exons using blastn. The sequence alignment output terminates at the exact end of the alignment. I would like to see some additional bases at the end of the subject sequence so as to see where the GT/AG sequence is in the genomic sequence. I cant seem to see an option in the blastn manual. I saw some options such as best_hit_overhang but it didn't give the desired output.
Thanks in advance
I am try to align RNAseq data to a genome to map the exons using blastn. The sequence alignment output terminates at the exact end of the alignment. I would like to see some additional bases at the end of the subject sequence so as to see where the GT/AG sequence is in the genomic sequence. I cant seem to see an option in the blastn manual. I saw some options such as best_hit_overhang but it didn't give the desired output.
Thanks in advance
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