We are looking to do some RNA-seq projects using Illumina for bacterial gene expression, and we are trying to decide between ribo-depleting with RiboZero and using Epicentre's ScriptSeq kits. Or my supervisor is curious about using DSN for ribo-depleting and normalization using the Illumina kits. We are not really looking particularly for rare transcripts, and it seems based on the papers that the standard is Ribo-Zero or some type of ribo-depletion kit, but I haven't seen or heard a lot about DSN treatment. Thus, I was just curious what people have found as the positives and negatives to each of the systems.
Thanks,
Kerry
Thanks,
Kerry
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