Tweet
Im working with paired-end RNA-seq data, and for my purpose I map the forward and reverse reads individually. What is the easiest way to assemle reads into a single alignment file while forcing the original direction to be preserved? So e.g. I want forward and reverse information of reads in final alignments.
-
-
You know that aligners that make .sams will natively figure out how to combined your paired end data so that you still know which read cam from which fastq, right?
If you really have to work with these bams, set the flags yourself to indicate which come from read1, and which come from read 2, then merge the .bams.
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment