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  • How to convert CRAM back to BAM using samtools

    Hello everyone,

    I just installed samtools v1.0 and want to test CRAM feature. I converted .BAM file to .CRAM and It was successful. Now I want to convert back .CRAM to .BAM but don't know how to do that. Does anyone know how to do that? Please show me. Thank you.

  • #2
    It's the same command, just use the -b option instead of -C.

    Comment


    • #3
      Originally posted by dpryan View Post
      It's the same command, just use the -b option instead of -C.
      Thank you for your quick response. I will try and let you know the result.

      Comment


      • #4
        Originally posted by dpryan View Post
        It's the same command, just use the -b option instead of -C.
        Hello Devon Ryan,
        Can you please help me check my command is correct or not?
        samtools view -T <fasta_path> -b -o <bam_file_path> <cram_file_path>.

        I ran this command and got the error 'Servname not supported for ai_socktype'

        Thank you for your time.

        Comment


        • #5
          Are you running this on amazon e3 or something like that? This error message will normally only happen if there's some issue regarding networking (generally receiving an IP when a name is expected or vice versa).

          Comment


          • #6
            Originally posted by dpryan View Post
            Are you running this on amazon e3 or something like that? This error message will normally only happen if there's some issue regarding networking (generally receiving an IP when a name is expected or vice versa).
            I will contact with my admin to fix this problem. Thank you very much.

            Comment

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