Hi,
Using a 10 pM pooled library of 6 indexed samples (4 from FFPE + 2 from fresh tissues) prepared with TruSeq Exome kit, I got this error message when running on MiSeq with no data generated (except the log files):
'Through-focus peak did not exceed SNR threshold (Illumina.Instrument.Bolt.Logic.AutoFocus.DarkSampleException)'
Do you have any idea what it means?
What could be the cause of this error?
Thanks !
Using a 10 pM pooled library of 6 indexed samples (4 from FFPE + 2 from fresh tissues) prepared with TruSeq Exome kit, I got this error message when running on MiSeq with no data generated (except the log files):
'Through-focus peak did not exceed SNR threshold (Illumina.Instrument.Bolt.Logic.AutoFocus.DarkSampleException)'
Do you have any idea what it means?
What could be the cause of this error?
Thanks !
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