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  • [NEWBIE] Interpretation of blastp results

    I'm extremely new to this and I apologize if this question seems naive. I created a database for virulence factors and did BLASTP.

    I got the the following values:

    qaccver saccver pident length mismatch gapopen qstart qend sstart send evalue bitscore

    However, according to my reference journal, I need "slen" or length of matching sequence and "qlen" or length of query sequence to be used in a formula to identify the homologous protein. The formula is:
    Ha = pident x slen/qlen

    Is "slen/qlen" equivalent to "length" already?

    or can I just use evalue and pident to identify the best match? Can you recommend a protocol for this?

    Thank you!

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