Hi, all!
Using DESeq, I generated estimated dispersion plot with my new RNA-seq data, and I had very different result from my previous one and other plots on the web. Also, strange result was obtained from the MA plot.
I tried 'htseq-count' with either 'intersection-strict' or 'union' options for counting, and both options gave me the same results.
Is there a problem with my counting result, or is it normal to have those pattern?
Using DESeq, I generated estimated dispersion plot with my new RNA-seq data, and I had very different result from my previous one and other plots on the web. Also, strange result was obtained from the MA plot.
I tried 'htseq-count' with either 'intersection-strict' or 'union' options for counting, and both options gave me the same results.
Is there a problem with my counting result, or is it normal to have those pattern?