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Old 01-11-2017, 11:25 AM   #1
PigletIA
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Default rRNA derived cDNA removal after library generation

I have a specific application that requires ~5ug total fragmented RNA input for an RNA-IP step, then library generation and removal of the rRNA derived cDNA. I saw the Clontech Pico Input Mammalian Strand-Specific RNA-Seq Libraries for Illumina Platforms from Picogram Inputs of Total RNA has components called ZapR and R-Probe for doing this, I'm just wondering if there is a home-brew version I can use for a more flexible application.
Thanks!
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Old 01-11-2017, 12:51 PM   #2
HESmith
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See this publication.
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Old 01-11-2017, 01:09 PM   #3
PigletIA
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Fantastic! Thanks a lot!
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