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Old 08-22-2011, 09:36 AM   #1
lvandiepen
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Default Illumina TruSeq resuspension Buffer

Hello,
Does anyone know what the resuspension buffer in the TruSeq RNA library prep kit contains/what the ingredients are? Is it comparable with 10 mM TRIS?

Thanks,
Linda
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Old 08-31-2011, 02:34 PM   #2
NextGenSeq
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I'm sure it also contains Tween-20. They recommend adding it to prevent the DNA sticking to the tube
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Old 02-23-2013, 06:47 AM   #3
marca
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10 mM Tris-HCl, pH 8.5
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Old 08-14-2013, 03:24 PM   #4
MAdkisson
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Is anyone certain about whether or not Tween-20 is involved?
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Old 08-15-2013, 06:12 AM   #5
NextGenSeq
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We resuspend all our libraries in Low TE with 0.1% Tween-20

The Tween-20 prevents libraries sticking to the microtubes.
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Old 01-03-2017, 12:45 PM   #6
linvei91
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Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?
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Old 01-03-2017, 03:14 PM   #7
jdk787
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Quote:
Originally Posted by linvei91 View Post
Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?
Yes it is.
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