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Old 04-21-2014, 11:06 AM   #1
Genohub
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Default 100 Gb Data/Day – Nextseq 500 Sequencing Services Now Available on Genohub

We're pleased to announce you can order NextSeq 500 sequencing services on Genohub.com today: https://genohub.com/shop-by-next-gen...48c30d3f93d505

We expect the highest interest to be centered around targeted sequencing (exome or custom regions) and fast RNA profiling. For exome studies, you can run between 1-12 samples in a single run and get back 4 Gb at 2 x75 or 5 Gb at a 2×100 read length. If you’re interested in RNA profiling at 10M reads per sample, you can multiplex between 12-36 samples together in a single run. A 1×75 cycle run takes as few as 11 hours to complete and 2×150 runs take ~29 hours.

For more details: http://blog.genohub.com/100-gb-of-da...le-on-genohub/
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Old 04-22-2014, 10:47 PM   #2
SNPsaurus
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Any idea if the NextSeq is more MiSeq or HiSeq-like in its tolerance for low-diversity reads (16S, small number of amplicons, etc)?
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Old 04-24-2014, 07:20 AM   #3
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The NextSeq is less tolerant than HiSeq for low-diversity reads. NextSeq uses a two channel SBS sequencing process. It's easier for current software versions to differentiate cross-talk matrix with 4 dyes. MiSeq is still the way to go for amplicon-seq, 16S. We've written a few words about it here:
https://genohub.com/ngs-instrument-guide/#illumina

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Old 04-24-2014, 08:28 AM   #4
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Good thoughts.
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