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Cheapest platform in terms of consumables (flow cells) Shaia Suki Wong General 4 02-24-2016 07:40 PM

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Old 01-10-2017, 06:44 AM   #1
LMM
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Default Best/ cheapest Platform for RNA-Seq

I am a MS student working with soil microbiome. I am looking for the presence of a gene family in soils under certain conditions and would like to use RNASeq to profile its expression in soil community. Gene is conserved in bacteria and fungi, so I will be analyzing the transcriptomes of both. Illumina HiSeq is off the table, but Im looking into Illumina MiSeq, IonTorrent, or Roche 454. Anyone have any insight?
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Old 01-10-2017, 06:52 AM   #2
GenoMax
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If you need volume sequencing with the lowest error rate then of the two remaining (454 is deprecated) MiSeq is the clear winner. If you don't have access to a local core then you can comparison shop at allseq.com or genohub.com.
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illumina miseq, rnaseq library, soil microbiome

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