Hi everyone,
Looking to get additional perspective on an issue driving me crazy. I am currently troubleshooting some library prep issues and am doing multiple extractions of one sample.
1 kit - MoBio ultraclean microbial DNA
1 kit - Qiagen DNeasy blood and tissue
1 CTAB + phenol chloroform
After the extractions I QC with nanodrop and qubit HS.
MoBio the results correlate nicely between the nanodrop and qubit. For the Qiagen and CTAB I get a tenfold difference in concentration.
I have done a gel and it appears to be the presence of RNA. I have already incorporated RNase A into the protocols. I double checked the efficacy of our stocks and they are working fine.
For the CTAB method the only thing I can think of is it might be contamination in the chloroform or the ethanol?
Thanks
Looking to get additional perspective on an issue driving me crazy. I am currently troubleshooting some library prep issues and am doing multiple extractions of one sample.
1 kit - MoBio ultraclean microbial DNA
1 kit - Qiagen DNeasy blood and tissue
1 CTAB + phenol chloroform
After the extractions I QC with nanodrop and qubit HS.
MoBio the results correlate nicely between the nanodrop and qubit. For the Qiagen and CTAB I get a tenfold difference in concentration.
I have done a gel and it appears to be the presence of RNA. I have already incorporated RNase A into the protocols. I double checked the efficacy of our stocks and they are working fine.
For the CTAB method the only thing I can think of is it might be contamination in the chloroform or the ethanol?
Thanks
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