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Does anyone use Fluidigm C1 instrument on cells using DNASeq script and final library prep for NGS? Our lab has followed their published protocol using a control cancer cell line & we haven't got good alignment of reads across all chromosomes and have run into some other technical issues that makes us think the WGA used on the C1 isn't that good. Part of the problem is that nearly all the published papers use C1 for RNA-Seq while there are very few DNA-Seq papers out there.
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Every WGA method isn't that good. -
Do you use the C1 NextGenSeq? Fluidigm gives the illustra WGA kit as their choice. When using that, it doesn't work to evenly amplify the template. Does anyone use another kit than the one Fluidigm recommends in their protocol or another process that would be better to perform DNA Seq on single cells?Comment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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