Hi everyone,
I was just wondering if anyone here has ever used a hotspot vcf in order to obtain homozygous reference genotypes.
I noticed that some calls that were called as "PASS" before using a hotspot are subsequently called "NOCALL" after using hotspot.
Has anyone encountered this?
Thanks,
Rabu
I was just wondering if anyone here has ever used a hotspot vcf in order to obtain homozygous reference genotypes.
I noticed that some calls that were called as "PASS" before using a hotspot are subsequently called "NOCALL" after using hotspot.
Has anyone encountered this?
Thanks,
Rabu