We are working on single-cell RNA seq using a version of Simone Picelli modified SMARTer method. Instead of Superscript II we were using Clontech's Primerscript. Unfortunately, the latest batches/ lots have been giving huge nonspecific products around 200-300bp. We switched from Superscript II because they had a problem with bacterial RNA contamination. Is anyone out there using alternate RT enzymes for the single-cell RNA seq protocols? Any response or advise would be greatly appreciated!
I'll try attaching our data below....
Thanks,
ply
I'll try attaching our data below....
Thanks,
ply
Comment