My questions are about amplicon sequencing.
I would like to sequence numerous PCR products on the Illumina GA.
I have about 50 amplicons that are my test group which I plan to scan on a set of 5 different RNA samples resulting in ~ 250 PCR products (amplicons).
1. Is there a recommended protocol for the preparation of the amplicons for sequencing on the Illumina GA?
2. Can I add the adpters directly to the PCR primers used to generate the amplicons?
3. What is the best way to barcode (tag) the different PCR amplicons coming from different RNA sample?
Best
Khen
I would like to sequence numerous PCR products on the Illumina GA.
I have about 50 amplicons that are my test group which I plan to scan on a set of 5 different RNA samples resulting in ~ 250 PCR products (amplicons).
1. Is there a recommended protocol for the preparation of the amplicons for sequencing on the Illumina GA?
2. Can I add the adpters directly to the PCR primers used to generate the amplicons?
3. What is the best way to barcode (tag) the different PCR amplicons coming from different RNA sample?
Best
Khen
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