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  • KEGG with pathview using CuffDiff data

    Hey everybody,

    I am a newbie in coding in Bioinformatic and I have a question.

    Using a HPC I perform a quality control and a read alignment with CuffLinks. At the end, I have a folder with CuffDiff. I open it with R using readCufflinks (library cummeRbund). And now I am trying to perform the metabolic pathways related to development (starch and sugar metabolism, Ethylene for example) but I don't know how to do it. I try with "pathview" but it doesn't work.

    Indeed, I have to find the kegg number or accession number but I don't know how to find it from the data of CuffDiff. Do you have any hints?
    Only hints that I find is on MicroArrays and it's not like me...

    Thanks for your help,
    Best regards.

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