I'm relatively new at working with RNA and I'm getting ready to make my first Library next week. Since I'm new to most these techniques and I want to be able to throw out anything that I feel might have been accidentally contaminated my standard operating procedure has been to make smaller aliqouts of all my reagents so I don't have to throw everything away if I make a mistake.
I was just considering aliqouting the AMPure XP magnetic beads for this reason but I have never worked with magnetic beads before. Other than vortexing the beads to make sure they are evenly suspended before aliqouting are there any considerations I need to be aware of? For example, I'm assuming it is OK to store them in standard RNA/DNA low binding tube eppendorf tubes?
I know this probably seems like a dumb question but the lab I'm in doesn't really have any molecular bio expertise and I'm learning everything from the product manuals, so any help would be greatly appreciated.
I was just considering aliqouting the AMPure XP magnetic beads for this reason but I have never worked with magnetic beads before. Other than vortexing the beads to make sure they are evenly suspended before aliqouting are there any considerations I need to be aware of? For example, I'm assuming it is OK to store them in standard RNA/DNA low binding tube eppendorf tubes?
I know this probably seems like a dumb question but the lab I'm in doesn't really have any molecular bio expertise and I'm learning everything from the product manuals, so any help would be greatly appreciated.
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