Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • My translated A.A seq longer than my original Nucleotide seq!! Could someone explain

    Hello everyone!

    I am in the process of identifying an uncharacterized protein, when I convert my nucleotide sequence to Amino Acid using blastx, I get a long Amino Acid sequence. I am not sure if I am doing something wrong or missing a step or there is a biological explanation for it that I don't know of.

    Nucleotide sequence / Accession Number X79334 region 333-548

    GTTATTGTGTTCGCCGTTTTGCTGACGGCTTCTTGTCTGATGGTCTCCTTTGCCAACAGCTTTACGCTGC
    TATTGCTGGACCGCGCCTGTCTTGGGTTGGCGCTGGACGGATTCTGGGCGATGTCGGCGTCGCTGACCAT
    GCGACTGGTTCCCGCGCGTACCGTGCCGAAAGCGCTGTCGGTGATTTTTGGCGCGGTCTCCATCGCGTTA
    GTGATC

    Amino Acid Sequence / Protein accession number ERH37120.1


    MNENIAEKFRADGVARPNWSAVFAVAFCVACLITVEFLPVSLLTPMAQDLGISEGIAGQSVTVTAFVAMF
    SSLFITQIIQATDRRYIVILFAVLLTASCLMVSFANSFTLLLLGRACLGLALGGFWAMSASLTMRLVPAR
    TVPKALSVIFGAVSIALVIAAPLGSFFGCXGISWSGRXALRPSAVMGVLC

    I am new to Bioinformatics, but I am fascinated by this developing field. I was researching my question and came across this amazing website. I hope someone can help.

  • #2
    Did you check whether any part of the amino acid sequence corresponds to your nucleotide sequence?

    Perhaps blastx translated the entire nucleotide sequence, 1-548, and not just the part annotated as a gene (333-548)? Would the length of the amino acid sequence be consistent with that?

    Comment


    • #3
      This is the full sequence of a matching protein that you're seeing.
      Your sequence translates to:
      VIVFAVLLTASCLMVSFANSFTLLLLDRACLGLALDGFWAMSASLTMRLVPARTVPKALSVIFGAVSIALVI
      And it was aligned to the segment starting "VILFAVLLTASCLMVSFANS".

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      68 views
      0 likes
      Last Post seqadmin  
      Working...
      X