hi ^_^
In the s_N_export.txt file, If the Match Position for one read is x, should the other end position of this read be x+75 both for "F" forward and "R" reverse strand match? (75 is the read length) Now we try x-75 for the reads matching "R" reverse strand and get some negative number position. So there must be mistake in x-75.
Thank you for your time!
In the s_N_export.txt file, If the Match Position for one read is x, should the other end position of this read be x+75 both for "F" forward and "R" reverse strand match? (75 is the read length) Now we try x-75 for the reads matching "R" reverse strand and get some negative number position. So there must be mistake in x-75.
Thank you for your time!
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