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Old 03-20-2019, 10:25 AM   #1
ssully
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Default genome assembly polisher that can use Sanger reads?

We have a PacBio final assembly that requires some polishing to fix e.g. erroneous stop codons. No Illumina reads at hand but we do have a set of high quality Sanger reads from an older version of the genome. Is there a tool like Racon but can use Sanger reads to correct small errors in an assembly?
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Old 06-27-2019, 09:55 AM   #2
JTH-Genome
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You could try to modify one of these error correction algorithms: http://wgs-assembler.sourceforge.net...index.php/PBcR
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