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Old 02-03-2015, 01:26 AM   #1
Simone78
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Location: Basel (Switzerland)

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Default EA1 protease treatment before WGA

Hi!

I have to do a WGA from few cells (10 or less) and I need to strip off all the proteins from the DNA before starting with the protocol. I came across a kit from ZyGEM which contains EA1 proteinase (+ Ak1 also, I think). This proteinase has the advantage that it gets completely inactivated by a short inactivation at 95 deg. (contrary to proteinase K which needs harsher conditions in terms of salt and denaturing agents), it works in common buffers and doesn´t need to be removed before proceeding with the following enzymatic reactions.
I think that EA1 is the same contained in some (maybe all!) WGA kits, such as the Picoplex from Rubicon Genomics.

My question is: does anybody know if any other company is selling the EA1 proteinase separately or do I really need to buy the kit?
Thanks in advance!
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