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Old 06-15-2015, 04:10 AM   #1
paumarc
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Location: Zagreb

Join Date: Feb 2015
Posts: 11
Default estimation of the expression changes

Hello everybody,

My colleges send me its pyrosequencing results to be analysed. the data that i got is a list of averages for different experiments (conditions) for a different genes

ex

Gene S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 CONTROL
XX1 86.038 79.980 83.187 87.194 87.959 69.002 80.041 73.444 83.024 82.699 86.927 83.454 78.727
XX2 86.808 82.174 87.807 78.769 89.416 79.372 79.746 87.551 77.332 84.987 87.896 84.957 87.184
XX3 95.332 97.720 96.260 96.553 95.989 97.200 96.499 97.579 96.745 96.454 97.803 96.703 91.181
XX4 36.937 34.742 33.443 40.455 37.312 39.778 37.982 34.913 39.422 39.443 34.303 39.174 40.547
XX5 21.317 21.628 21.940 23.089 22.348 21.640 22.073 22.051 21.843 22.137 22.554 21.947 26.284
XX6 39.516 38.141 49.775 49.492 46.538 44.756 38.753 29.113 41.901 34.615 45.711 41.394 46.708
XX7 43.969 45.714 44.218 42.237 44.505 43.324 46.178 47.514 45.741 46.358 44.233 46.013 60.407
XX8 96.073 86.632 96.787 95.297 97.420 96.737 87.502 95.741 96.095 97.132 96.661 97.162 97.208
XX9 39.802 40.546 53.222 57.733 40.821 38.104 44.429 45.639 47.922 0.000 49.030 49.347 66.852



I used edgeR to see which genes has a significant change and i get two genes in two samples. Now my boss is interested to know which genes has an expression change above 50
%. Can some one tell me how to estimate this percentage? there is some easy way?

thanks

pau
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