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Old 10-01-2018, 09:14 AM   #1
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Location: Israel

Join Date: May 2011
Posts: 4
Default Strand information for alignment of KAPA mRNA HyperPrep Kit data

I am looking on data generated with KAPA mRNA HyperPrep Kit. this library prep is stranded. When I run Salmon/Histat2 for alignment, it asks whether the data is strandedF/strandedR/unstranded. I ran all options and they give slightly different results.
Is the option I should use the Stranded F? Also, if anyone can direct me to an explanation on how you get stranded information that would be helpful.

Thank you!!!
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