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Old 09-25-2012, 07:55 AM   #1
Volklor
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Location: Pacific Northwest

Join Date: Sep 2010
Posts: 13
Default velvetg "irregular sequence" error

Hello,
I've been trying to run velvetg for a de novo assembly project and have encountered an error I haven't seen before. I'm not sure where to start looking to troubleshoot this. Has anyone seen this before?

[757.395968] Sequence 34000000 / 134431570
[762.312525] Sequence 35000000 / 134431570
[767.392179] Sequence 36000000 / 134431570
[773.210880] Sequence 37000000 / 134431570
[778.708129] Sequence 38000000 / 134431570
[783.844449] Sequence 39000000 / 134431570
[788.606782] Sequence 40000000 / 134431570
[793.582053] Sequence 41000000 / 134431570
[793.648029] Irregular sequence file: are you sure your Sequence and Roadmap file come from the same source?
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Old 09-25-2012, 06:36 PM   #2
Torst
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Location: The University of Melbourne, AUSTRALIA

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Default

It looks like you may be re-using the same folder over again for different assemblies and there is inconsistent files in it. Try running velveth + velvetg with a new folder name, or delete the existing folder completely.
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Old 10-01-2012, 08:41 AM   #3
Volklor
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Location: Pacific Northwest

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Default Thanks!

Thanks Torst - changing the folder name solved it.
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