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Old 08-26-2008, 06:58 AM   #1
minky81
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Default Adapter sequence

Hi,

Can someone please tell me what is an "Adapter sequence" and what is its purpose?

Thanks
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Old 11-02-2008, 10:56 PM   #2
ECO
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These are any short piece of DNA of known sequence that one adds to the ends of their unknown DNA of interest, usually for the purpose of eventually placing a sequencing primer there.
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Old 04-02-2010, 08:53 AM   #3
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Quote:
Originally Posted by ECO View Post
These are any short piece of DNA of known sequence that one adds to the ends of their unknown DNA of interest, usually for the purpose of eventually placing a sequencing primer there.
Hi,

I have illumina multiplex paired end data of 75bp. The insert size 300-400bp including primer and adapter sequences. I want to check whether my data contains adapter sequence or not for that i used SSAAHA2 and the result file shows matched sequences at different positions like 66th bp to 40th bp, 75th bp to 60th bp. But mostly from the right side of the read.

I want to know can we adapter sequences on both sides of illumina reads.

What if we have 15 bases of adapter sequence out of 20 bases on the right side like from 50th to 65th bp? and then we have other sequence as well that makes up readlength 75bp. Will that be adapter sequence or not?
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