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Questions about hardware specifications for analysis sallusti10 Illumina/Solexa 7 02-09-2010 11:39 AM

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Old 02-10-2014, 06:22 AM   #1
alunem
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Default MiSeqDx specifications

What are the specifications of the FDA-cleared version of the MiSeq. As I understood, it's a downgrade version of it (as it had to be locked since the beginning of FDA application processing), but what exactly?
- what is the expected ouput (number of reads, lenght...)
- what files are going to be available for the user? (are we going to have access to fastq files? bcl?)

Well, it's not easy to find these information on illumina's website.

Thanks!
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Old 02-10-2014, 06:30 AM   #2
GenoMax
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http://res.illumina.com/documents/pr...instrument.pdf
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Old 02-10-2014, 06:35 AM   #3
alunem
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Thanks, this answers my first question. Any idea about files available after sequencing processed?
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Old 02-10-2014, 06:40 AM   #4
GenoMax
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You may want to check with your local FAS (who can in turn check with someone here in the US, if the instrument is not available in France).

My feeling (speculation) is the output may be some kind of a summary report saying such and such mutation is present/absent. No data may be directly accessible. Someone who has a MiSeqDX perhaps can provide additional information.
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Old 02-10-2014, 09:13 AM   #5
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Wonder how they dealt with the well known carryover contamination issue. This would be a major problem for viral or tumor sequencing.
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