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Old 05-28-2019, 04:17 AM   #1
ecSeq Bioinformatics
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Lightbulb How can unique molecular identifiers (UMIs) help to reduce quantitative biases?

Maybe you have already heard about UMIs and how they can help to reduce errors and quantitative biases in your Next-Generation Sequencing (NGS) experiment.
But do you know how they work and how you should handle them bioinformatically after sequencing?

Here we will shortly introduce these molecular tags, which are added to the DNA fragments of your sample.

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Last edited by ecSeq Bioinformatics; 06-04-2019 at 04:59 AM.
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Old 05-28-2019, 09:23 AM   #2
luc
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Quote:
... we will shortly introduce these newly developed molecular tags ...
???

UMIs have been used since at least 2013, then sequenced on an old GAII:
"Exploring local immunological adaptation of two stickleback ecotypes by experimental infection and transcriptome-wide digital gene expression analysis."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579235/
Please see the TrueQuant mentions in the paper.

Last edited by luc; 05-28-2019 at 09:25 AM.
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Old 06-04-2019, 03:27 AM   #3
ecSeq Bioinformatics
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Thanks for the information, luc! We'll change it on the website.
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