SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
A first look at Illumina’s new NextSeq 500 AllSeq Vendor Forum 108 07-09-2018 02:23 AM
Dual indexing construct for MiSeq - where to put the barcode for index2? Vinz Illumina/Solexa 50 12-12-2014 02:19 PM
no dual indices on NextSeq 500 (yet) SeqNerd Illumina/Solexa 9 10-20-2014 11:06 AM
Custom amplicon sequencing with dual indexing tickseq Illumina/Solexa 2 06-27-2013 09:46 AM
96 or 384 Nextera-style dual indexing pmiguel Illumina/Solexa 3 06-27-2012 09:49 AM

Reply
 
Thread Tools
Old 06-10-2014, 08:49 AM   #1
bryanbriney
Junior Member
 
Location: San Diego

Join Date: May 2014
Posts: 8
Default Dual indexing on NextSeq

Illumina announced updates to the Prep tab in BaseSpace that enable a couple of missing features for NextSeq users -- dual indexing and custom indexes. Also, we just received some new Mid-output kits and the Accessory Box now contains an additional 'dual index primer' reagent, which is to be added to well #18 when performing a run with dual indexes.

http://blog.basespace.illumina.com/2...pace-prep-tab/
bryanbriney is offline   Reply With Quote
Old 06-19-2014, 06:59 AM   #2
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default

Yep. They've changed the way dual indexing is done on on the NextSeq. BP10 is the primer to read the i5 index and needs to be added to #18 for all dual index runs. Our FAS told us paired end turnaround is carried out even on single read conformations in order to read the second index. The reason they switched is because the lack of dark cycles before the index read improves the read quality (less (pre)phasing) and therefore gives better assignment. The software must reverse compliment the i5 read because it's now reading from the other direction.
TonyBrooks is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:04 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO