SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: High-throughput genotyping of hop (Humulus lupulus L.) utilising diversity ar Newsbot! Literature Watch 0 08-16-2011 10:00 AM
PubMed: Large-scale sequencing of plant small RNAs. Newsbot! Literature Watch 0 08-07-2011 05:12 AM
PubMed: Deep sequencing of viroid-derived small RNAs from grapevine provides new insi Newsbot! Literature Watch 0 11-06-2009 02:00 AM
PubMed: Deep-sequencing of plant viral small RNAs reveals effective and widespread ta Newsbot! Literature Watch 0 08-12-2009 05:00 AM

Reply
 
Thread Tools
Old 05-08-2010, 02:00 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,439
Default PubMed: High-throughput sequencing of Hop stunt viroid-derived small RNAs from cucumb

Syndicated from PubMed RSS Feeds

High-throughput sequencing of Hop stunt viroid-derived small RNAs from cucumber leaves and phloem.

Mol Plant Pathol. 2010 May 1;11(3):347-59

Authors: Martinez G, Donaire L, Llave C, Pallas V, Gomez G

Small RNA (sRNA)-guided processes, referred to as RNA silencing, regulate endogenous and exogenous gene expression. In plants and some animals, these processes are noncell autonomous and can operate beyond the site of initiation. Viroids, the smallest self-replicating plant pathogens known, are inducers, targets and evaders of this regulatory mechanism and, consequently, the presence of viroid-derived sRNAs (vd-sRNAs) is usually associated with viroid infection. However, the pathways involved in the biogenesis of vd-sRNAs are largely unknown. Here, we analyse, by high-throughput pyrosequencing, the profiling of the Hop stunt viroid (HSVd) vd-sRNAs recovered from the leaves and phloem of infected cucumber (Cucumis sativus) plants. HSVd vd-sRNAs are mostly 21 and 22 nucleotides in length and derived equally from plus and minus HSVd RNA strands. The widespread distribution of vd-sRNAs across the genome reveals that the totality of the HSVd RNA genome contributes to the formation of vd-sRNAs. Our sequence data suggest that viroid-derived double-stranded RNA functions as one of the main precursors of vd-sRNAs. Remarkably, phloem vd-sRNAs accumulated preferentially as 22-nucleotide species with a consensus sequence over-represented. This bias in size and sequence in the HSVd vd-sRNA population recovered from phloem exudate suggests the existence of a selective trafficking of vd-sRNAs to the phloem tissue of infected cucumber plants.

PMID: 20447283 [PubMed - in process]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:32 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO