SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Complete characterization of the edited transcriptome of the mitochondrion of Newsbot! Literature Watch 0 10-18-2011 02:00 AM
PubMed: Characterization of the horseweed (Conyza canadensis) transcriptome using GS- Newsbot! Literature Watch 0 08-18-2010 02:11 AM
PubMed: Analysis of microRNA transcriptome by deep sequencing of small RNA libraries Newsbot! Literature Watch 0 05-13-2010 02:00 AM
PubMed: Characterization of the Zoarces viviparus liver transcriptome using massively Newsbot! Literature Watch 0 08-04-2009 05:00 AM
PubMed: Rapid transcriptome characterization for a nonmodel organism using 454 pyrosq Newsbot! Literature Watch 0 02-13-2008 07:38 AM

Reply
 
Thread Tools
Old 10-14-2008, 05:00 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,439
Default PubMed: In-depth characterization of the microRNA transcriptome in a leukemia progres

Syndicated from PubMed RSS Feeds

Related Articles In-depth characterization of the microRNA transcriptome in a leukemia progression model.

Genome Res. 2008 Oct 10;

Authors: Kuchenbauer F, Morin RD, Argiropoulos B, Petriv OI, Griffith M, Heuser M, Yung E, Piper J, Delaney A, Prabhu AL, Zhao Y, McDonald H, Zeng T, Hirst M, Hansen CL, Marra MA, Humphries RK

MicroRNAs (miRNAs) have been shown to play important roles in physiological as well as multiple malignant processes, including acute myeloid leukemia (AML). In an effort to gain further insight into the role of miRNAs in AML, we have applied the Illumina massively parallel sequencing platform to carry out an in-depth analysis of the miRNA transcriptome in a murine leukemia progression model. This model simulates the stepwise conversion of a myeloid progenitor cell by an engineered overexpression of the nucleoporin 98 (NUP98)-homeobox HOXD13 fusion gene (ND13), to aggressive AML inducing cells upon transduction with the oncogenic collaborator Meis1. From this data set, we identified 307 miRNA/miRNA* species in the ND13 cells and 306 miRNA/miRNA* species in ND13+Meis1 cells, corresponding to 223 and 219 miRNA genes. Sequence counts varied between two and 136,558, indicating a remarkable expression range between the detected miRNA species. The large number of miRNAs expressed and the nature of differential expression suggest that leukemic progression as modeled here is dictated by the repertoire of shared, but differentially expressed miRNAs. Our finding of extensive sequence variations (isomiRs) for almost all miRNA and miRNA* species adds additional complexity to the miRNA transcriptome. A stringent target prediction analysis coupled with in vitro target validation revealed the potential for miRNA-mediated release of oncogenes that facilitates leukemic progression from the preleukemic to leukemia inducing state. Finally, 55 novel miRNAs species were identified in our data set, adding further complexity to the emerging world of small RNAs.

PMID: 18849523 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:34 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO