SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
TrueSeq PCR free POOR LIGATION efficiencies Degraz Illumina/Solexa 3 04-08-2015 05:03 AM
TruSeq DNA PCR-free vs NANO DNA kit mbk0asis Illumina/Solexa 8 05-17-2014 08:15 AM
PCR-Free Libraries & Bioinformatic PCR Duplicate detection? aeonsim Bioinformatics 3 10-23-2013 01:36 PM
The NEXTflex™ PCR-Free DNA Sequencing Kit Reduces Bias in NGS Library Prep Bioo Scientific Vendor Forum 10 12-20-2012 07:10 AM
bias in merging sequence data? quinne5 RNA Sequencing 0 11-01-2012 09:17 AM

Reply
 
Thread Tools
Old 09-30-2016, 12:34 PM   #1
hudja
Junior Member
 
Location: planet Earth

Join Date: Sep 2016
Posts: 1
Question Batch bias while merging TrueSeq PCR-free and Nano data

Hi All,

We are starting a new sequencing project of human DNA samples. Part of our sample was already sequenced using Illumina TrueSeq PCR-free kit. Now, it turned out that part of the new samples does not qualify for PCR-free protocol and we were suggested to switch them to TrueSeq Nano protocol. In addition, we have been advised that PCR-free protocol could have a big failure rate during library prep currently, up to 15%. The issue seems to be connected to changes in kit chemistry and affects many labs worldwide.

However, I am a little bit worried about the possible extent of batch bias arising when processing/merging data from two different protocols (PCR-free and Nano). Does anybody here have this kind of experience?

Thank you in advance!
hudja is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:24 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO