Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Seq-cap Bioinformatic workflow help Giorgio C Bioinformatics 0 02-02-2012 01:45 AM
RNA-Seq: Comparative Analysis of RNA-Seq Alignment Algorithms and the RNA-Seq Unified Newsbot! Literature Watch 3 07-31-2011 07:08 PM
pileup mapping quality cap and SNPs? MatthewVC Bioinformatics 2 06-06-2011 07:29 AM
tell me the different between two version cap? robertorun Bioinformatics 3 07-07-2010 09:23 PM
Deletion detection on seq-cap experiments Tom Bair 454 Pyrosequencing 0 10-31-2008 10:10 AM

Thread Tools
Old 10-21-2010, 01:07 PM   #1
Junior Member
Location: Montreal

Join Date: May 2009
Posts: 3
Question 5' cap and RNA-Seq

How does the presence of a 5' cap on mRNA affect RNA-Seq processing? (I'm especially interested in Illumina sequencing.) Is the cap removed during mRNA preparation or fragmentation? If the cap persists, does it interfere with PCR, attachment to the flow cell, or sequencing? Does poor detection of capped fragments contribute to the tailing off of read coverage at 5' ends of transcripts?

I haven't found any useful information about this through Pubmed or Google, but I bet someone here knows the answers.
ian.d.reid is offline   Reply With Quote

5' cap, illumina, mrna, rna-seq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:27 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO