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  • Error joing segment hits in tophat

    Can anyone offer advice on what to do about this error -11, or even what it means:

    [Fri Mar 11 15:54:38 2011] Beginning TopHat run (v1.2.0)
    -----------------------------------------------
    [Fri Mar 11 15:54:38 2011] Preparing output location tophat_out/cDNA/organ/
    [Fri Mar 11 15:54:38 2011] Checking for Bowtie index files
    [Fri Mar 11 15:54:38 2011] Checking for reference FASTA file
    [Fri Mar 11 15:54:38 2011] Checking for Bowtie
    Bowtie version: 0.12.7.0
    [Fri Mar 11 15:54:38 2011] Checking for Samtools
    Samtools Version: 0.1.12a
    [Fri Mar 11 15:54:40 2011] Checking reads
    min read length: 21bp, max read length: 991bp
    format: fasta
    [Fri Mar 11 15:54:41 2011] Mapping reads against Anocarol2.0.61_toplevel with Bowtie
    [Fri Mar 11 15:55:05 2011] Joining segment hits
    [FAILED]
    Error: Segment join failed with err = -11
    Last edited by Wallysb01; 03-11-2011, 03:43 PM.

  • #2
    We are experiencing exactly the same error mentioned in the post above running a particular batch of 454 RNAseq. The same version of TopHat (v1.2.0) was used.
    I could not find a list with details of TopHat error codes. That would be useful for troubleshooting.

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