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I was hoping that someone might know of a good probe design software. My method is sort of based off of the oligo ligation assay and MLPA but it's not exactly either of those. I just want to be able to have something that if I input a sequence with a SNP, it will return the upstream hybridization oligo and downstream hybridization oligo to a SNP site. I have attached a photo to describe what I mean. I realize this might not be the best forum to post this in, but I don't know where else to ask. My goal would be to make hybridization oligos within a specific size range, close Tm, and 50/50ish GC content. I can do this by hand for a couple but I would like to expand my project to a couple of hundred sites. If anyone has any program suggestions I would appreciate it. Ideally something a dummy (like me) could use or at least a program with some tutorials. I have looked into Probemaker but even that seems awfully complicated. Even if the program isn't free I would look into it.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM