We have no experience in Sanger sequencing of small products, 70-100 bp. So far, we've figured out that we'll need BigDye v1.1, a new spectral calibration, maybe a different POP version, and likely a new way to purify the PCR products because Qiagen columns cut off around 100 bp. We'd like to do the cleanup in plate format. In short, we need advice, big time!
Any suggestions, comments, resources out there? Perhaps it is best to just outsource?
Thanks.
Barry
Any suggestions, comments, resources out there? Perhaps it is best to just outsource?
Thanks.
Barry
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