I am looking to determine if the Hepatitis C Virus genomic sequences (variant population structure) between two samples is different to a statistically significant degree.
If the nucleotide substitution or error rate is 1 in 1000 nucleotides and my target is 1665 nucleotides in length, could i determine a difference in the two populations by sampling 100 clones from each population using traditional sanger sequencing ?
or would we need to use Nex Gen Sequencing ?
If we need to NGS, the what depth of coverage would we need to have sufficient power to determine a difference ?
I really appreciate any response!!
If the nucleotide substitution or error rate is 1 in 1000 nucleotides and my target is 1665 nucleotides in length, could i determine a difference in the two populations by sampling 100 clones from each population using traditional sanger sequencing ?
or would we need to use Nex Gen Sequencing ?
If we need to NGS, the what depth of coverage would we need to have sufficient power to determine a difference ?
I really appreciate any response!!
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