Help needed regarding designing primers

[discoveradnan]

Hello everyone!

I have background knowledge of designing primers but I am facing difficulty in finding a way to design primers specifically for Indels and structural changes of a diseased gene. So is there any database available out there which can give me information about that? So i can design primers accordingly. Any help will be really appreciated. Thanks

[nucacidhunter]

A document on subject is attached:
BIO450 Primer Design Tutorial 2006.pdf

I am sure you can find other tutorials on the web and YouTube.

[discoveradnan]

A document on subject is attached:
[ATTACH]4089[/ATTACH]

I am sure you can find other tutorials on the web and YouTube.

nucacidhunter thanks for you reply.
I am specifically looking for a database from where i can find annotated indels and structural changes of any diseased genes. So that i can design primers accordingly. If you know any, kindly do let me know. Thanks