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Old 03-15-2010, 05:20 AM   #1
severin
Genome Informatics Facility
 
Location: Iowa @isugif

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Default A scaling normalization method for differential expression analysis of RNA-seq data

Genome Biology 2010, 11:R25 doi:10.1186/gb-2010-11-3-r25
Mark D Robinson (mrobinson@wehi.edu.au)
Alicia Oshlack (oshlack@wehi.edu.au)

The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq is likely to become the platform of choice for interrogating steady state RNA. In order to discover biologically important changes in expression, we show that normalization continues to be an essential step in the analysis. We outline a simple and effective method for performing normalization and show dramatically improved results for inferring differential expression in simulated and publicly available data sets.

http://genomebiology.com/2010/11/3/R25
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Old 09-09-2010, 11:09 PM   #2
daniel.liu
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thank you for severin provide this information
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