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Old 06-28-2011, 07:00 AM   #261
spacedninja
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Location: USA

Join Date: Jun 2011
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Default Hello

Hi, I'm a lab tech who's recently been told I'll be doing all of our 454 analysis work. Figured this place as well as other faqs and everything else would be a good resource. Will probably post my first question very soon. (and I used to think I was alright with computers)
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Old 06-30-2011, 02:02 AM   #262
thurisaz
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Hi all,

I'm a PhD student in a plant genetics lab. Being a linux user with some programming experience, I seem to have become the "bioinformatics guy" in the lab. We've already successfully used SHORE to identify two mutants from a mutagenesis via resequencing; now I'm trying to prepare to analyse some RNAseq data from one of our mutants.
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Old 06-30-2011, 07:12 AM   #263
husamia
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Hi all,
I am an application specialist. I have Bachelor of Science in Biomedical Engineering and 2nd Major in Pure Mathematics for curiosity reasons. I havn't found direct benefit of it but I might be wrong. I am my own mentor for the good and bad. I am involved in developing highthroughput screening projects since 2006. strong practical experience in linux being mathematically inclined. my dad bought me a computer in early 80's when I was less than 10 years old. I remember it had MSX, enough said about me.

Last edited by husamia; 06-30-2011 at 07:16 AM.
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Old 06-30-2011, 08:45 AM   #264
LLG
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Location: Georgia

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Default Hey-

I am new member, and began working with the 454 GS Junior the last year. I am a technician here in Athens, GA. I do all the benchwork and upkeep with the Jr, but hope to do some analysis soon. I think this forum is very useful and I decided to join. I have done shotgun libraries for the large FLX and now do rapid libraries and some paired end runs on the Jr. Hope to do more studies soon.
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Old 06-30-2011, 09:18 AM   #265
tarkvara
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Location: Toronto

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Default Introduction

Hi. I'm one of the developers of the Savant Genome Browser. If anybody has questions or comments about Savant, feel free to contact me.
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Old 06-30-2011, 09:40 AM   #266
WSW
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Default Hi

Hi, just getting into resequencing and found SEQanswers quite helpful, so I wanted to signup.
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Old 07-03-2011, 05:08 PM   #267
pfudd
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Hi. I'm the bioinformatics guy/sysadmin/programmer in our lab. We're studying metagenomics, which means tons of sequence data. Yaaaay!
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Old 07-06-2011, 12:03 PM   #268
xomicx
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Hi,
XomicX is a service company providing efficient, cost effective outsourcing solutions in all disciplines of bioinformatics, biostatistics, computational biology and medical informatics.

XomicX offers a flexible online workspace where experts and seekers of service can come together and collaborate. In this virtual space, diverse parties can specify the services they require, identify and contact each other regarding particular projects.

We invite you to visit our website www.xomicx.com. Please send me an email if you have any question.
xomicx
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Old 07-07-2011, 05:39 AM   #269
agent.hm
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Hi,

I am currently working on GAII. hoping to learn from y'all!
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Old 07-07-2011, 01:17 PM   #270
richard_tilde
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Herro!
For months now, my google searches on RNA-seq topics have consistently resulted in 1) finding threads from this forum OR 2) finding links to seqanswers threads in helpful comments at various blogs.

٩๏̯͡๏)۶ I don't know why I waited so long to register! ٩๏̯͡๏)۶
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Old 07-08-2011, 03:35 AM   #271
sajancr
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Hi everyone,
I'm a Sajan Raju from India, working as Bioinformatician. Analyzing metagenomics sequence data from 454 titanium FLX platform. Also analyzing a complete genome of pseudomnas strain....


Cheers!
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Old 07-08-2011, 06:31 AM   #272
cessbronson
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Location: São Paulo - Brazil

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Hello there,

I'm from Brazil and I'm currently working on a de novo assembly of a rat transcriptome. My data was obtained using a Solid sequencer and the we used BioScope to make the alignment and we're trying to use cufflinks to estimate the transcripts and FPKM. So far we managed to run cufflinks doing a reference-based run and we obtained fairly good results. But when I tried the de novo way I had all sorts of issues, which is what brought me here.

I hope I find my answers and contribute with whatever I can to the community.

Best regards
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Old 07-08-2011, 06:42 AM   #273
Aaron_Sarver
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Location: Minnesota

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Hey all,
I have decide that I need to become more aware of what/ how other people are analyzing seqeuncing data. So far I have been developing approaches to deal with RNA-seq, Exome reseqeuncing, CHIP-seq and Transposon Insertion mapping for a number of labs a the University of minnesota. Google searching on the various problems I have enocountered using the open source tools has led me to this forum on multiple occasions so i hope to get involved as it seems to be a needed and useful resource.

Aaron
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Old 07-09-2011, 05:17 AM   #274
Syntrichia
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Hello all,
My name's Carmine, based in a University in southern Italy, I have no experience in NGS. I am a plant systematist interested in phylogeny, particularly of bryophytes, I have experience in Sanger sequencing of nuclear and cpDNAs. I am wondering if NGS would be a cheaper option to Sanger sequencing for my type of work.
Regards.
Carmine
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Old 07-09-2011, 07:56 AM   #275
artful dodger
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Hi!

I am a statistician and bioinformatics masters student working on de novo sequencing data of a bacterium. The data I've been working on was generated using a 454 system. I hope I can cope with the data. Genomic data is fascinatingly complex, simply unputdownable
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Old 07-09-2011, 09:22 AM   #276
elena.or
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Hallo, I am new in the forum.
I am working as a Postdoc in Germany but I soon will start working for a breeding company. I am interested in the new genotyping platforms and I hope to get good advice :-) !
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Old 07-10-2011, 04:17 PM   #277
seqnextgen
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Hi, All:

I have been in this next gen business for a few years and also for work a company for a living. However, I won't represent anything but myself here. Like to have great knowledge exchange with everyone here.

--seqnextgen
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Old 07-10-2011, 09:59 PM   #278
Inam
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Hi all,
Myself Inam, from India. I am involved with a group working on plant molecular biology. Came across this forum, liked it a lot. I will mostly be working on SOLiD data analysis.
Cheers,
Inam
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Old 07-11-2011, 08:38 AM   #279
Sarah_Tulin
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Hi, I'm a postdoc at MBL, Woods Hole, MA, USA. I'm working with an Illumina HiSeq, planning to do transcriptomics. I've already found lots of helpful information here and will continue to use this site.
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Old 07-11-2011, 08:53 AM   #280
tboothby
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Hello,
I am a graduate student at the University of Maryland. I am interested in the regulation of alternative splicing (and other aspects of PTR) during development. To this end I am working on de novo transcriptome assembly.

The lab I am working in does not have a lot of sequencing/bioinfromatics experience and I have already found this forum quite helpful.

I have just completed my first Illumina GAII deepseq run, and assembled my sequences using Trinity. Now I am trying to make sense of all the data...
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